Review





Similar Products

sod1  (ABclonal Biotechnology)
94
ABclonal Biotechnology sod1
Sod1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sod1/GAP43+Rabbit+mAb/10__1016_slash_j__ctmp__2026__200304-127-83-85
Average 94 stars, based on 1 article reviews
sod1 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
sod  (Proteintech)
94
Proteintech sod
Sod, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sod1/SOD1+Fusion+Protein/10__1016_slash_j__aquaculture__2026__744568-88-26-28
Average 94 stars, based on 1 article reviews
sod - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
rabbit polyclonal anti sod1  (ABclonal Biotechnology)
95
ABclonal Biotechnology rabbit polyclonal anti sod1
Rabbit Polyclonal Anti Sod1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sod1/SOD1+Rabbit+pAb/pmc13533451-1267-0-4
Average 95 stars, based on 1 article reviews
rabbit polyclonal anti sod1 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
sod a0274  (ABclonal Biotechnology)
95
ABclonal Biotechnology sod a0274
Sod A0274, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sod1/SOD1+Rabbit+pAb/pm42413764-60-18-16
Average 95 stars, based on 1 article reviews
sod a0274 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
ho 1  (ABclonal Biotechnology)
95
ABclonal Biotechnology ho 1
Ho 1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sod1/SOD1+Rabbit+pAb/pm41793617-55-14-15
Average 95 stars, based on 1 article reviews
ho 1 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
anti rabbit sod1 antibody  (ABclonal Biotechnology)
95
ABclonal Biotechnology anti rabbit sod1 antibody
( A ) Venn diagram depicting the number of unique and shared proteins expressed in the Gas muscle of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). KO, knockout. ( B ) PCA plot of the Gas muscle from Pax +/+ , Myh7 fl/+ (red) and Pax3 cre/+ , Myh7 Δ/fl (green) mice at P0, where each dot represents a biological replicate ( n = 6). ( C ) Volcano plot showing up-regulated and down-regulated proteins in the Gas muscle from Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). ( D ) Pathway analysis (Reactome database) of Gas muscle proteins from Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0. ( E ) Heatmap depicting the NRF2 pathway target protein expression in the Gas muscle of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). ( F and G ) Representative Western blots for <t>SOD1,</t> GPx1, Prdx2, Prdx1, catalase, and α-tubulin (loading control) using protein lysates from the Gas muscle of P0 Pax +/+ , Myh7 fl/+ , Pax3 cre/+ , Myh7 Δ/fl , and Pax7 icre/+ , Myh7 Δ/fl mice (F) and their densitometry (G) ( n = 5). ( H to M ) Representative Western blots for NRF2, P-NRF2, and α-tubulin (loading control) using Gas and TA muscle protein lysates of P0 Pax +/+ , Myh7 fl/+ , Pax3 cre/+ , Myh7 Δ/fl , and Pax7 icre/+ , Myh7 Δ/fl mice (H and I) and their densitometry (J to M) ( n = 5). ( N and O ) Label-free quantitation (LFQ) intensity of DRP1 and FIS1 by mass spectrometry on P0 Gas muscle protein lysates of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice ( n = 6). ( P ) Heatmap depicting the expression of mitochondrial proteins in the Gas muscle of P0 Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice ( n = 6). Data are presented as means ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
Anti Rabbit Sod1 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sod1/SOD1+Rabbit+pAb/pmc13232616-437-161-165
Average 95 stars, based on 1 article reviews
anti rabbit sod1 antibody - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
rabbit monoclonal anti superoxide dismutase 1 sod1  (Thermo Fisher)
96
Thermo Fisher rabbit monoclonal anti superoxide dismutase 1 sod1
Oxidative stress markers in the study population, stratified by obesity presence, before and after non-surgical periodontal treatment. Total ROS (A and G), cytosolic superoxide (B and H) and mitochondrial superoxide (C and I) in leukocytes, and protein levels of <t>SOD1</t> (D and J) in PBMCs normalized to the loading control actin with corresponding WB images (E), and serum total antioxidant capacity (F and K). Data are presented as box and whisker plots and were compared using two-factor analysis of variance (ANOVA) followed by post hoc tests. ** P < .01 when comparing baseline vs 12 wk. # P < .05 when comparing groups with vs without obesity. DCFH, diclorodihidrofluoresceína; dHE, dihydroethidium; MitoSOX, Mitochondrial Superoxide Indicator; ns, not significant; PBMCs, peripheral blood mononuclear cells; ROS, reactive oxygen species; SOD1, superoxide dismutase 1.
Rabbit Monoclonal Anti Superoxide Dismutase 1 Sod1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sod1/Superoxide+Dismutase/pmc12966717-109-20-42
Average 96 stars, based on 1 article reviews
rabbit monoclonal anti superoxide dismutase 1 sod1 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


( A ) Venn diagram depicting the number of unique and shared proteins expressed in the Gas muscle of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). KO, knockout. ( B ) PCA plot of the Gas muscle from Pax +/+ , Myh7 fl/+ (red) and Pax3 cre/+ , Myh7 Δ/fl (green) mice at P0, where each dot represents a biological replicate ( n = 6). ( C ) Volcano plot showing up-regulated and down-regulated proteins in the Gas muscle from Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). ( D ) Pathway analysis (Reactome database) of Gas muscle proteins from Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0. ( E ) Heatmap depicting the NRF2 pathway target protein expression in the Gas muscle of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). ( F and G ) Representative Western blots for SOD1, GPx1, Prdx2, Prdx1, catalase, and α-tubulin (loading control) using protein lysates from the Gas muscle of P0 Pax +/+ , Myh7 fl/+ , Pax3 cre/+ , Myh7 Δ/fl , and Pax7 icre/+ , Myh7 Δ/fl mice (F) and their densitometry (G) ( n = 5). ( H to M ) Representative Western blots for NRF2, P-NRF2, and α-tubulin (loading control) using Gas and TA muscle protein lysates of P0 Pax +/+ , Myh7 fl/+ , Pax3 cre/+ , Myh7 Δ/fl , and Pax7 icre/+ , Myh7 Δ/fl mice (H and I) and their densitometry (J to M) ( n = 5). ( N and O ) Label-free quantitation (LFQ) intensity of DRP1 and FIS1 by mass spectrometry on P0 Gas muscle protein lysates of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice ( n = 6). ( P ) Heatmap depicting the expression of mitochondrial proteins in the Gas muscle of P0 Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice ( n = 6). Data are presented as means ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Journal: Science Advances

Article Title: The skeletal muscle slow myosin heavy chain regulates mammalian metabolic homeostasis through the NRF2 pathway

doi: 10.1126/sciadv.aed2478

Figure Lengend Snippet: ( A ) Venn diagram depicting the number of unique and shared proteins expressed in the Gas muscle of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). KO, knockout. ( B ) PCA plot of the Gas muscle from Pax +/+ , Myh7 fl/+ (red) and Pax3 cre/+ , Myh7 Δ/fl (green) mice at P0, where each dot represents a biological replicate ( n = 6). ( C ) Volcano plot showing up-regulated and down-regulated proteins in the Gas muscle from Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). ( D ) Pathway analysis (Reactome database) of Gas muscle proteins from Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0. ( E ) Heatmap depicting the NRF2 pathway target protein expression in the Gas muscle of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice at P0 ( n = 6). ( F and G ) Representative Western blots for SOD1, GPx1, Prdx2, Prdx1, catalase, and α-tubulin (loading control) using protein lysates from the Gas muscle of P0 Pax +/+ , Myh7 fl/+ , Pax3 cre/+ , Myh7 Δ/fl , and Pax7 icre/+ , Myh7 Δ/fl mice (F) and their densitometry (G) ( n = 5). ( H to M ) Representative Western blots for NRF2, P-NRF2, and α-tubulin (loading control) using Gas and TA muscle protein lysates of P0 Pax +/+ , Myh7 fl/+ , Pax3 cre/+ , Myh7 Δ/fl , and Pax7 icre/+ , Myh7 Δ/fl mice (H and I) and their densitometry (J to M) ( n = 5). ( N and O ) Label-free quantitation (LFQ) intensity of DRP1 and FIS1 by mass spectrometry on P0 Gas muscle protein lysates of Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice ( n = 6). ( P ) Heatmap depicting the expression of mitochondrial proteins in the Gas muscle of P0 Pax +/+ , Myh7 fl/+ and Pax3 cre/+ , Myh7 Δ/fl mice ( n = 6). Data are presented as means ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: The antibodies used for Western blotting were as follows: anti-mouse MyHC-IIa antibody (SC-71, Developmental Studies Hybridoma Bank) at 1:10 dilution; anti-mouse MyHC-IIb antibody (BFF3, Developmental Studies Hybridoma Bank) at 1:10; anti-mouse alpha-tubulin antibody (30021290, Cell Signaling Technology) at 1:10,000; anti-rabbit GAPDH antibody (PAB932Mu01, Cloud-Clone Corp) at 1:10,000; anti-rabbit Akt antibody (4641, Cell Signaling Technology) at 1:1000; anti-rabbit P-Akt S473 (4640, Cell Signaling Technology) at 1:1000; anti-rabbit Histone H3 antibody (Ab1741, Abcam) at 1:10,000; anti-rabbit troponin T1 antibody (NBP238855, Novus Biologicals) at 1:5000; anti-rabbit PGC-1α antibody (A12348, ABclonal; 66369, Proteintech; and 2178S, Cell Signaling Technology) at 1:2000, 1:2000, and 1:1000, respectively; anti-mouse total OXPHOS cocktail (ab110413, Abcam) at 1:1000; anti-rabbit DRP1 antibody (A21968, ABclonal) at 1:2000; anti-rabbit FIS1 antibody (A19666, ABclonal) at 1:2000; anti-rabbit mitofusin1 antibody (A9880, ABclonal) at 1:2000; anti-rabbit mitofusin2 antibody (A12771, ABclonal) at 1:2000; anti-rabbit GLUT4 (MA5-17176, Thermo Fisher Scientific) at 1:1000; anti-rabbit NRF2 antibody (A0674, ABclonal and 98020000, Proteintech) at 1:2000; anti-rabbit P-NRF2 antibody (AP1133, ABclonal) at 1:2000; anti-rabbit SOD1 antibody (A0274, ABclonal) at 1:2000; anti-rabbit Prdx1 antibody (A13524, ABclonal) at 1:2000; anti-rabbit Gpx1 antibody (A1110, ABclonal) at 1:1000; anti-rabbit catalase antibody (A11220, ABclonal) at 1:2000; anti-rabbit KEAP1 antibody (A1820, ABclonal) at 1:1000; anti-rabbit Tom20 antibody (HPA011582, Merck) at 1:2500, anti-rabbit VDAC1 antibody ( Ab154856 , Abcam) at 1:2500; anti-rabbit pDRP1 (S616) antibody (AP0849, ABclonal) at 1:2500; and anti-rabbit OPA1 antibody (A9833, ABclonal) at 1:2500.

Techniques: Knock-Out, Expressing, Western Blot, Control, Quantitation Assay, Mass Spectrometry

( A to C ) Representative Western blots for troponin T1, GAPDH, NRF2, KEAP1, catalase, GPx1, Prdx1, SOD1, and α-tubulin (loading control) using TA and soleus muscle protein lysates from 6-month-old WT mice (A) and their densitometry (B and C) ( n = 5). ( D to H ) Representative Western blots for P-NRF2, NRF2, and α-tubulin (loading control) using Gas muscle protein lysates of 2- and 6-month-old Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice (D) and their densitometry (E to H) ( n = 5). ( I to K ) Representative Western blots for total NRF2 and H3A in the nuclear fraction and total NRF2 and GAPDH in the cytoplasmic fraction ( I ), using protein lysates from the Gas muscle of P0 Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice and their densitometric quantification (J and K) ( n = 4). ( L to Q ) Representative Western blots for SOD1, GPx1, Prdx2, Prdx1, catalase, carbonylated proteins, and α-tubulin (loading control) using protein lysates from the Gas muscle of 2- and 6-month-old Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice (L and O) and their densitometry (M, N, P, and Q) ( n = 5). ( R to T ) Cross sections through the Gas of Pax +/+ , Myh7 fl/+ (R to Rb), and Pax7 icre/+ , Myh7 Δ/fl (S to Sb) mice at 12 months of age, labeled by immunofluorescence for γ-H2AX (red) and DAPI (blue), and quantification of γ-H2AX+ cells (T). Scale bars, 100 μm (S) and 10 μm (Sb). ( U to X ) Representative Western blots for γ-H2AX, cleaved caspase-3, p21, IGF-1, and α-tubulin (loading control) using protein lysates from the Gas muscle of 2- and 6-month-old Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice (U) and their densitometry (V to X) ( n = 5). Data are presented as means ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Journal: Science Advances

Article Title: The skeletal muscle slow myosin heavy chain regulates mammalian metabolic homeostasis through the NRF2 pathway

doi: 10.1126/sciadv.aed2478

Figure Lengend Snippet: ( A to C ) Representative Western blots for troponin T1, GAPDH, NRF2, KEAP1, catalase, GPx1, Prdx1, SOD1, and α-tubulin (loading control) using TA and soleus muscle protein lysates from 6-month-old WT mice (A) and their densitometry (B and C) ( n = 5). ( D to H ) Representative Western blots for P-NRF2, NRF2, and α-tubulin (loading control) using Gas muscle protein lysates of 2- and 6-month-old Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice (D) and their densitometry (E to H) ( n = 5). ( I to K ) Representative Western blots for total NRF2 and H3A in the nuclear fraction and total NRF2 and GAPDH in the cytoplasmic fraction ( I ), using protein lysates from the Gas muscle of P0 Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice and their densitometric quantification (J and K) ( n = 4). ( L to Q ) Representative Western blots for SOD1, GPx1, Prdx2, Prdx1, catalase, carbonylated proteins, and α-tubulin (loading control) using protein lysates from the Gas muscle of 2- and 6-month-old Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice (L and O) and their densitometry (M, N, P, and Q) ( n = 5). ( R to T ) Cross sections through the Gas of Pax +/+ , Myh7 fl/+ (R to Rb), and Pax7 icre/+ , Myh7 Δ/fl (S to Sb) mice at 12 months of age, labeled by immunofluorescence for γ-H2AX (red) and DAPI (blue), and quantification of γ-H2AX+ cells (T). Scale bars, 100 μm (S) and 10 μm (Sb). ( U to X ) Representative Western blots for γ-H2AX, cleaved caspase-3, p21, IGF-1, and α-tubulin (loading control) using protein lysates from the Gas muscle of 2- and 6-month-old Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice (U) and their densitometry (V to X) ( n = 5). Data are presented as means ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: The antibodies used for Western blotting were as follows: anti-mouse MyHC-IIa antibody (SC-71, Developmental Studies Hybridoma Bank) at 1:10 dilution; anti-mouse MyHC-IIb antibody (BFF3, Developmental Studies Hybridoma Bank) at 1:10; anti-mouse alpha-tubulin antibody (30021290, Cell Signaling Technology) at 1:10,000; anti-rabbit GAPDH antibody (PAB932Mu01, Cloud-Clone Corp) at 1:10,000; anti-rabbit Akt antibody (4641, Cell Signaling Technology) at 1:1000; anti-rabbit P-Akt S473 (4640, Cell Signaling Technology) at 1:1000; anti-rabbit Histone H3 antibody (Ab1741, Abcam) at 1:10,000; anti-rabbit troponin T1 antibody (NBP238855, Novus Biologicals) at 1:5000; anti-rabbit PGC-1α antibody (A12348, ABclonal; 66369, Proteintech; and 2178S, Cell Signaling Technology) at 1:2000, 1:2000, and 1:1000, respectively; anti-mouse total OXPHOS cocktail (ab110413, Abcam) at 1:1000; anti-rabbit DRP1 antibody (A21968, ABclonal) at 1:2000; anti-rabbit FIS1 antibody (A19666, ABclonal) at 1:2000; anti-rabbit mitofusin1 antibody (A9880, ABclonal) at 1:2000; anti-rabbit mitofusin2 antibody (A12771, ABclonal) at 1:2000; anti-rabbit GLUT4 (MA5-17176, Thermo Fisher Scientific) at 1:1000; anti-rabbit NRF2 antibody (A0674, ABclonal and 98020000, Proteintech) at 1:2000; anti-rabbit P-NRF2 antibody (AP1133, ABclonal) at 1:2000; anti-rabbit SOD1 antibody (A0274, ABclonal) at 1:2000; anti-rabbit Prdx1 antibody (A13524, ABclonal) at 1:2000; anti-rabbit Gpx1 antibody (A1110, ABclonal) at 1:1000; anti-rabbit catalase antibody (A11220, ABclonal) at 1:2000; anti-rabbit KEAP1 antibody (A1820, ABclonal) at 1:1000; anti-rabbit Tom20 antibody (HPA011582, Merck) at 1:2500, anti-rabbit VDAC1 antibody ( Ab154856 , Abcam) at 1:2500; anti-rabbit pDRP1 (S616) antibody (AP0849, ABclonal) at 1:2500; and anti-rabbit OPA1 antibody (A9833, ABclonal) at 1:2500.

Techniques: Western Blot, Control, Labeling, Immunofluorescence

( A ) Schematic depicting the sulforaphane dosing strategy starting from 16 weeks. ( B to F ) Body weight (grams) and muscle weight (TA, Gas, Quad, and soleus) (milligrams) of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice at 6 months of age ( n = 5 to 6). ( G to Gc ) Gas muscle cross sections labeled by immunofluorescence for laminin (red) and DAPI (blue) of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice at 6 months of age and quantification of the proportion of myofibers grouped according to myofiber area (Gb) and average myofiber area (μm 2 ) (Gc) through the Gas ( n = 4). Scale bar, 100 μm (Ga). ( H to Ia ) Ex vivo contractility quantifying the absolute and maximal absolute force of TA and Gas in DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice ( n = 4). ( J and K ) Treadmill running distance and speed of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice ( n = 6). ( L to V ) Representative Western blots for P-NRF2, NRF2, KEAP1 (Gas and TA), SOD1, GPx1, Prdx1, catalase, MyHC-IIa, MyHC-IIb, PGC-1α, mitochondrial complex, carbonylated proteins (Gas), and α-tubulin (loading control) using protein lysates of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice (L, O, S, and T) and their densitometry (M, N, P to R, U, and V) ( n = 4). ( W ) Grip strength quantification of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice normalized to body weight ( n = 6). ( X to Ya ) GTT and ITT of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice and their area under the curve ( n = 5 to 6). ( Z ) Quad muscle ATP level quantification of DMSO- or sulforaphane-treated Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice ( n = 5). Data are presented as means ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Journal: Science Advances

Article Title: The skeletal muscle slow myosin heavy chain regulates mammalian metabolic homeostasis through the NRF2 pathway

doi: 10.1126/sciadv.aed2478

Figure Lengend Snippet: ( A ) Schematic depicting the sulforaphane dosing strategy starting from 16 weeks. ( B to F ) Body weight (grams) and muscle weight (TA, Gas, Quad, and soleus) (milligrams) of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice at 6 months of age ( n = 5 to 6). ( G to Gc ) Gas muscle cross sections labeled by immunofluorescence for laminin (red) and DAPI (blue) of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice at 6 months of age and quantification of the proportion of myofibers grouped according to myofiber area (Gb) and average myofiber area (μm 2 ) (Gc) through the Gas ( n = 4). Scale bar, 100 μm (Ga). ( H to Ia ) Ex vivo contractility quantifying the absolute and maximal absolute force of TA and Gas in DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice ( n = 4). ( J and K ) Treadmill running distance and speed of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice ( n = 6). ( L to V ) Representative Western blots for P-NRF2, NRF2, KEAP1 (Gas and TA), SOD1, GPx1, Prdx1, catalase, MyHC-IIa, MyHC-IIb, PGC-1α, mitochondrial complex, carbonylated proteins (Gas), and α-tubulin (loading control) using protein lysates of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice (L, O, S, and T) and their densitometry (M, N, P to R, U, and V) ( n = 4). ( W ) Grip strength quantification of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice normalized to body weight ( n = 6). ( X to Ya ) GTT and ITT of DMSO- or sulforaphane-treated Pax7 icre/+ , Myh7 Δ/fl mice and their area under the curve ( n = 5 to 6). ( Z ) Quad muscle ATP level quantification of DMSO- or sulforaphane-treated Pax +/+ , Myh7 fl/+ and Pax7 icre/+ , Myh7 Δ/fl mice ( n = 5). Data are presented as means ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: The antibodies used for Western blotting were as follows: anti-mouse MyHC-IIa antibody (SC-71, Developmental Studies Hybridoma Bank) at 1:10 dilution; anti-mouse MyHC-IIb antibody (BFF3, Developmental Studies Hybridoma Bank) at 1:10; anti-mouse alpha-tubulin antibody (30021290, Cell Signaling Technology) at 1:10,000; anti-rabbit GAPDH antibody (PAB932Mu01, Cloud-Clone Corp) at 1:10,000; anti-rabbit Akt antibody (4641, Cell Signaling Technology) at 1:1000; anti-rabbit P-Akt S473 (4640, Cell Signaling Technology) at 1:1000; anti-rabbit Histone H3 antibody (Ab1741, Abcam) at 1:10,000; anti-rabbit troponin T1 antibody (NBP238855, Novus Biologicals) at 1:5000; anti-rabbit PGC-1α antibody (A12348, ABclonal; 66369, Proteintech; and 2178S, Cell Signaling Technology) at 1:2000, 1:2000, and 1:1000, respectively; anti-mouse total OXPHOS cocktail (ab110413, Abcam) at 1:1000; anti-rabbit DRP1 antibody (A21968, ABclonal) at 1:2000; anti-rabbit FIS1 antibody (A19666, ABclonal) at 1:2000; anti-rabbit mitofusin1 antibody (A9880, ABclonal) at 1:2000; anti-rabbit mitofusin2 antibody (A12771, ABclonal) at 1:2000; anti-rabbit GLUT4 (MA5-17176, Thermo Fisher Scientific) at 1:1000; anti-rabbit NRF2 antibody (A0674, ABclonal and 98020000, Proteintech) at 1:2000; anti-rabbit P-NRF2 antibody (AP1133, ABclonal) at 1:2000; anti-rabbit SOD1 antibody (A0274, ABclonal) at 1:2000; anti-rabbit Prdx1 antibody (A13524, ABclonal) at 1:2000; anti-rabbit Gpx1 antibody (A1110, ABclonal) at 1:1000; anti-rabbit catalase antibody (A11220, ABclonal) at 1:2000; anti-rabbit KEAP1 antibody (A1820, ABclonal) at 1:1000; anti-rabbit Tom20 antibody (HPA011582, Merck) at 1:2500, anti-rabbit VDAC1 antibody ( Ab154856 , Abcam) at 1:2500; anti-rabbit pDRP1 (S616) antibody (AP0849, ABclonal) at 1:2500; and anti-rabbit OPA1 antibody (A9833, ABclonal) at 1:2500.

Techniques: Labeling, Immunofluorescence, Ex Vivo, Western Blot, Control

Oxidative stress markers in the study population, stratified by obesity presence, before and after non-surgical periodontal treatment. Total ROS (A and G), cytosolic superoxide (B and H) and mitochondrial superoxide (C and I) in leukocytes, and protein levels of SOD1 (D and J) in PBMCs normalized to the loading control actin with corresponding WB images (E), and serum total antioxidant capacity (F and K). Data are presented as box and whisker plots and were compared using two-factor analysis of variance (ANOVA) followed by post hoc tests. ** P < .01 when comparing baseline vs 12 wk. # P < .05 when comparing groups with vs without obesity. DCFH, diclorodihidrofluoresceína; dHE, dihydroethidium; MitoSOX, Mitochondrial Superoxide Indicator; ns, not significant; PBMCs, peripheral blood mononuclear cells; ROS, reactive oxygen species; SOD1, superoxide dismutase 1.

Journal: International Dental Journal

Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways

doi: 10.1016/j.identj.2026.109472

Figure Lengend Snippet: Oxidative stress markers in the study population, stratified by obesity presence, before and after non-surgical periodontal treatment. Total ROS (A and G), cytosolic superoxide (B and H) and mitochondrial superoxide (C and I) in leukocytes, and protein levels of SOD1 (D and J) in PBMCs normalized to the loading control actin with corresponding WB images (E), and serum total antioxidant capacity (F and K). Data are presented as box and whisker plots and were compared using two-factor analysis of variance (ANOVA) followed by post hoc tests. ** P < .01 when comparing baseline vs 12 wk. # P < .05 when comparing groups with vs without obesity. DCFH, diclorodihidrofluoresceína; dHE, dihydroethidium; MitoSOX, Mitochondrial Superoxide Indicator; ns, not significant; PBMCs, peripheral blood mononuclear cells; ROS, reactive oxygen species; SOD1, superoxide dismutase 1.

Article Snippet: Twenty-five micrograms of protein were separated by SDS-PAGE, transferred to nitrocellulose membranes, blocked, and incubated with the following specific antibodies: rabbit monoclonal anti-superoxide dismutase 1 (SOD1) (Cat# PA5-27240, RRID:AB_2544716), rabbit polyclonal anti-IRE1α (Cat# PA1-46027, RRID:AB_2262265), mouse monoclonal anti-CHOP (Cat# MA1-250, RRID:AB_2292611) from Thermo Fisher Scientific; rabbit polyclonal anti-GRP78 (Cat# ab21685, RRID:AB_2119834), mouse monoclonal anti-ATF6 (Cat# ab122897, RRID:AB_10899) from Abcam; rabbit polyclonal anti-eukaryotic initiation factor 2 phospho (p-eIF2α) (Innovative Research Cat# 44-728G, RRID:AB_15000); mouse monoclonal anti-actin (Cell Signaling Technology Cat# 3700, RRID:AB_2242334) or rabbit polyclonal anti-actin (Sigma-Aldrich Cat# A5060, RRID:AB_476738).

Techniques: Control, Whisker Assay